PRAME Knockout cell line (THP-1)

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PRAME gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PRAME gene knockout PRAME Knockout cell line (THP-1) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

Catalog#

YKO-HT1259

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Related Popular Products

Wild-type Cell Lines

Human Monocytes Cell Line(THP-1)
inquiry More details

Catalog#: YC-D011

Size: 1*10^6

Instruction:

Gene Knockout kit

EZ-editor™Gene Knockout kit
inquiry More details

Catalog#: MY-001

Size: -

Instruction:

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium
inquiry More details

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

KO Cell Lines

PRAME Knockout cell line (A-375)
inquiry More details

Organism: Human

Gene Name: PRAME

Gene ID: 23532

Size: 1*10^6

Catalog#: YKO-H1073

YKO-H1073(A-375)
PRAME Knockout cell line (HCT 116)
inquiry More details

Organism: Human

Gene Name: PRAME

Gene ID: 23532

Size: 1*10^6

Catalog#: YKO-H723

YKO-H723(HCT 116)
PRAME Knockout cell line (HeLa)
inquiry More details

Organism: Human

Gene Name: PRAME

Gene ID: 23532

Size: 1*10^6

Catalog#: YKO-XN49564

YKO-XN49564(HeLa)

Related Popular Products

Wild-type Cell Lines

Human Monocytes Cell Line(THP-1)

Price (USD): -

Catalog#: YC-D011

Size: 1*10^6

Instruction:

inquiry More details

Gene Knockout kit

EZ-editor™Gene Knockout kit

Price (USD): 998

Catalog#: MY-001

Size: -

Instruction:

inquiry More details

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium

Price (USD): -;-

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

inquiry More details

KO Cell Lines

PRAME Knockout cell line (A-375)

Organism: Human

Gene Name: PRAME

Gene ID: 23532

Size: 1*10^6

Catalog#: YKO-H1073

inquiry More details
PRAME Knockout cell line (HCT 116)

Organism: Human

Gene Name: PRAME

Gene ID: 23532

Size: 1*10^6

Catalog#: YKO-H723

inquiry More details
PRAME Knockout cell line (HeLa)

Organism: Human

Gene Name: PRAME

Gene ID: 23532

Size: 1*10^6

Catalog#: YKO-XN49564

inquiry More details

PRAME Knockout cell line (THP-1)

Subscribe Us